Figure 2 | Scientific Reports

Figure 2

From: Therapeutic potential of digitoflavone on diabetic nephropathy: nuclear factor erythroid 2-related factor 2-dependent anti-oxidant and anti-inflammatory effect

Figure 2

High glucose-mediated mesangial cell hyperplasia and inflammation can be antagonized by digitoflavone induced Nrf2.

(A) Expression of NF-κB pathway proteins was assessed by immunoblot analysis in SV40-Mes13 cells incubated in NG, HG, or HG + digitoflavone DMEM media for 24 h. (B) Expression of NF-κB downstream genes was assessed by real-time PCR analysis in SV40-Mes13 cells. (C) Expression of TGF-β1 and downstream proteins was assessed by immunoblot analysis in SV40-Mes13 cells. (D) Cell growth of SV40-Mes13 cells incubated in NG, HG, or HG + digitoflavone DMEM media were monitored in real-time for 30 h (upper panel = average; lower panel = average with error). (E) Cell hyperplasia and death were assessed by Ki67 immunolabeling or Hoechst 33258 staining. All data represent the mean ± SD of triplicate independent experiments. *P < 0.05 vs. NG group. #P < 0.05 vs. HG group.

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