Figure 5 | Scientific Reports

Figure 5

From: Parallel damage in mitochondrial and lysosomal compartments promotes efficient cell death with autophagy: The case of the pentacyclic triterpenoids

Figure 5

Ligand-interactions of triterpenoids in mimetic membranes.

(a) The analysis of cell membrane permeability was based on the release of carboxyfluorescein - CF. The percentage of CF-release was calculated in a time (i) and dose-dependent manner (ii). (b) DPOC, di-oleoyl-phosphocholine (C18∶1) bilayer was submitted to dynamic analysis of membrane interaction. Chemical structures of BA and OA (upper panel). Color coding: C (cyan), O (red) and polar H (white). The arrows indicate the chirality centers relevant for (C-i). Snapshot of the simulated system after 300 ns showing both BA and OA (van der Walls spheres) and the phospholipid bilayer depicted in gray. (c) Time evolution of the distance between triterpenoids and the bilayer center (i); in a second independent run (ii); and when the structure of both BA and OA was modified (iii) by inverting the chirality of the carbon atoms indicated by arrows in (B, upper triterpenoids structures). The dashed line represents the bilayer headgroups region. All results were obtained from at least three independent experiments and expressed as mean values ± standard error.

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