Figure 5
TNF-α could be a mediator of bryostatin mediated HIV-1 reactivation in human astrocytes.
(a) U-87 cells (2.5 × 106) were transiently transfected with pTNF-Luc, cultured in the presence of bryostatin (100 ng/ml) or prostratin (10 μg/ml) for 16 h and assayed for luciferase activity (mean ± S.D. of three experiments performed in triplicate; *p ≤ 0.05). (b) U-87 cells (2.5 × 106) transiently transfected with pLTR-Luc were treated with TNF-α and with supernatants from U-87 cells pretreated with bryostatin (100 ng/ml) during 16 h and assayed for luciferase activity (mean ± S.D. of three experiments performed in triplicate; (mean ± S.D. of three experiments performed in triplicate; *p ≤ 0.05, **p < 0.01). (c) Levels of TNF-α in the supernatants of U-87 cells treated during 24 h with bryostatin were measured by ELISA. The mean values (mean ± S.D.) of three independent experiments are shown (**p < 0.01).
