Figure 4 | Scientific Reports

Figure 4

From: A Novel Intravital Imaging Window for Longitudinal Microscopy of the Mouse Ovary

Figure 4

Physiological response of the ovary to hormonal stimulation.

(a–e) Stereomicroscope images and perfusion maps of the mouse ovary undergoing hormonal changes upon injection of pregnant mare’s serum gonadotropin (PMSG; injected at day 2) and human chorionic gonadotropin (hCG; injected 48 h after PMSG, at day 4). Asterix indicates the same ovarian follicle at different time points; arrow denotes the same blood vessel. Calibration bars denote color-coded intensity range (0–255) which corresponds to blood flow map of each time point. (f) Hematoxylin and eosin-stained histological section of the ovary showing corpora lutei formed after ovulation. Asterix denote corpora lutea. a-e were imaged in-vivo in the imaging window.

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