Figure 3
Effects of anti-Tmem106a on macrophage activation.
(a) Freshly prepared mouse macrophages (4 × 106 cells/well) were treated with isotype control IgG (dotted line), anti-Tmem106a (filled histograms) or LPS (solid black line) for 20 h. The cells were then stained with FITC-conjugated antibodies against murine MHC class II, CD86, CD80 and CD69, followed by flow cytometric analysis. Results are representatives of three independent experiments. (b) The results are expressed as mean ± SD from three independent experiments (*p < 0.05, **p < 0.01, ***p < 0.001). (c, d and e) Mouse macrophages from peritoneum (thioglycollate-elicited or not) and RAW264.7 were treated with isotype control IgG, anti-Tmem106a or LPS for 40 h. Concentrations of TNF-α, IL-1β, IL-6, CCL2, IL-10 and NO in the cultural supernatants were determined. The results are expressed as mean ± SD from three independent experiments (*p < 0.05, **p < 0.01, ***p < 0.001). (f) Mouse macrophages were treated with or without isotype control IgG, anti-Tmem106a, LPS or IL-4 (10 ng/ml) for 24 h. The levels of iNOS and arginase-1 were analyzed by western blot. Full-size images of western blots are shown in Supplementary Figure S5.
