Figure 4 | Scientific Reports

Figure 4

From: Transmembrane protein 106a activates mouse peritoneal macrophages via the MAPK and NF-κB signaling pathways

Figure 4

Knockdown of Tmem106a attenuates pro-inflammatory cytokine release induced by anti-Tmem106a.

Mouse macrophages were transfected with siControl RNA or siTmem106a RNA for 24 h, followed by analysis of the expression of Tmem106a protein by (a) Western blot or (b and c) flow cytometry. Full-size images of western blots are shown in Supplementary Figure S6. (d and e) Mouse macrophages from peritoneum were pretreated with siControl or siTmem106a-1/2 for 24 h, followed by incubation with isotype IgG control, anti-Tmem106a or LPS for 40 h. Concentrations of TNF-α, IL-1β, IL-6 and CCL2 in the cultural supernatants were determined using an ELISA kit. The results are expressed as mean ± SD from three independent experiments (*p < 0.05, **p < 0.01).

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