Figure 5
Anti-Tmem106a activates MAPK signals.
(a) Mouse macrophages were serum-starved for 24 h and thereafter exposed to isotype IgG (lanes 1–5), anti-Tmem106a (lanes 6–10) or LPS (lanes 11–15) for the periods of time indicated. Then the levels of phosphorylated MAPK and total MAPK were assessed by Western blot with specific antibodies. Full-size images of western blots are shown in Supplementary Figure S7. (b and c) Mouse thioglycollate-elicited peritoneal macrophages were pretreated with DMSO, U0126, SP600125, SB203580 for 1 h followed by stimulation with anti-tmem106a, isotype IgG and LPS for 24 h, The levels of CCL2, TNF-α, IL-6 and IL-1β in the cultural supernatants were determined by ELISA. The results are expressed as mean ± SD from three independent experiments (*p < 0.05, **p < 0.01, ***p < 0.001).
