Figure 6 | Scientific Reports

Figure 6

From: Transmembrane protein 106a activates mouse peritoneal macrophages via the MAPK and NF-κB signaling pathways

Figure 6

Anti-Tmem106a induces activation of NF-κB and STAT1 signals.

(a) Mouse macrophages were serum-starved for 24 h and thereafter exposed to isotype IgG (lanes 1–5), anti-Tmem106a (lanes 6–10) or LPS (lanes 11–15) for the periods of time indicated. Then, the levels of total and phosphorylated NF-κB p65, IKKα/β, STAT1 and STAT6 were assessed by western blot with specific antibodies. Full-size images of western blots are shown in Supplementary Figure S8. (b) For confocal laser-scanning microscopy, viable naïve peritoneal macrophages on glass slides were treated with control IgG, anti-Tmem106a or LPS for 30 min, followed by treatment with FITC-labeled goat anti-mouse NF-κB p65 antibody for 12 h at 4 °C and DAPI stainning. The slides were observed under a Leica confocal.

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