Figure 7
From: Characterization of the honeybee AmNaV1 channel and tools to assess the toxicity of insecticides
Fenvalerate induced tail currents in oocytes expressing AmNaV1.
The presence of 10 μM fenvalerate induced a tail current in oocytes co-expressing AmNaV1 and its TipE regulatory subunit. (a) The amplitude of the tail current was highly dependent on the number of depolarizations imposed before the recording (pulses from −80 mV to −20 mV repeated at a frequency of 100 Hz). (b) The amplitude of the tail current was also dependent on the concentration of fenvalerate in the extracellular medium. The red dotted line represents the fit of the tail current at 10 μM. (c) The number of conditioning pulses (CP) required to attain the maximal amplitude of the tail current in the presence of 10 μM fenvalerate was then determined (n = 3). (d) The fraction of channels modified by fenvalerate (M) was calculated using equation 1 in the supplemental methods and plotted as a function of the dose applied using 1700 conditioning pulses. The data was then fitted with a Hill curve in order to extract the Hill coefficient (h = 1.18) and the half maximal effective concentration (EC50 = 290 nM). The error bars represent the SEM.
