Figure 4
From: Hypoxia-inducible miR-182 enhances HIF1α signaling via targeting PHD2 and FIH1 in prostate cancer
miR-182 enhances the expression levels of HIF1α.
(a) Western blot analysis of HIF1α protein levels in DU145 and PC-3 cells transfected with miR-182 mimics and control oligos (NC). (b,c) Western blot analysis of HIF1α, PHD2 and FIH1 protein levels in DU145 (b) and PC-3 (c) cells transfected with miR-182 mimics and control oligos (NC) in the presence of hypoxia-mimetic DFO. (d,e) RT-PCR analysis of HIF1α (d) and VEGF (e) mRNA levels in DU145 and PC-3 cells transfected with miR-182 mimics and control oligos (NC). (f) Western analysis of HIF1α, PHD2 and FIH1 protein expression in PC-3 cells transfected with miR-182 antagomir (anti-182) after the induction of cellular hypoxia. (g) Western analysis of HIF1α, PHD2 and FIH1 protein expression in PC-3 cells transfected with miR-182 mimics or miR-182 antagomir (anti-182) in a normoxic environment. Data are mean ± SEM of three independent experiments. *p < 0.05, **p < 0.01. Full-length blots are presented in Supplementary Fig. S6.
