Figure 5
From: Hypoxia-inducible miR-182 enhances HIF1α signaling via targeting PHD2 and FIH1 in prostate cancer
miR-182 positively regulates prostate tumor angiogenesis.
(a) RT-PCR analysis of miR-182 levels in PC-3 cells infected with MDH1 (PC-3-MDH1) and MDH1-182 retrovirus (PC-3-MDH1-182). (b,c) In vivo matrigel plug assay. Matrigel mixed with PC-3-MDH1 or PC-3-MDH1-182 cells were injected into BALB/cA-nu/nu nude mice. The gross morphology (b) and hemoglobin content (c) of matrigel plugs were shown. (d) RT-PCR analysis of miR-182 levels in PC-3 cells transfected with miR-182 inhibitor or inhibitor negative control (NC). (e,f) In vivo matrigel plug assay. Matrigel mixed with PC-3 cells transfected with miR-182 inhibitor or inhibitor NC were injected into BALB/cA-nu/nu nude mice. The corresponding gross morphology (e) and hemoglobin content (f) of matrigel plugs were shown. Data are mean ± SEM (n = 6). *p < 0.05, **p < 0.01.
