Figure 2
Spatiotemporal analysis of extraparasitic HSP101.
(a) Quantification of HSP101-mCherry localization throughout a synchronized infection at 4 h intervals according to four categories indicated by representative images (top). The localization categories are: punctate cytoplasmic (white), additional periphery (light brown), one tubular extension (light blue) and two or more tubular extensions (dark blue). White outlines, erythrocyte; green, parasite cytoplasm; red, HSP101-mCherry. The proportions of extraparasitic HSP101-mCherry are indicated for six time points (n = 100 per time point) of the 24 h asexual blood-stage cycle. (b) Quantification of tubular length in relation to the red blood cell (RBC) diameter indicated by representative images (top). The length categories are: ≤0.5 RBC diameter (light brown), 0.5–1 RBC diameter (light blue) and ≥1 RBC diameter (dark blue). White outlines, erythrocyte; green, parasite cytoplasm; red, HSP101-mCherry. Length distributions of extraparasitic HSP101-mCherry are indicated for the same six time points (n = 100 per time point) as in (a).
