Table 2 Comparison of the Tm mapping and culture/sequencing results starting from bacterial colonies.

Difference Value (D	No. of samples	vs. Conventional culture method			vs. Sequencing method
Difference Value (D	No. of samples	No. of matches	No. of broad matches	No. of mismatches	No. of matches	No. of broad matches	No. of mismatches
0.0 ≤ D ≤ 0.28	108	102 (2^a)	2	4	106	2	0
0.28 < D ≤ 0.5	22	16	1	5	19	1	2
0.5 < D^b	10	6	0	4	6	0	4

^aThe number of matches at the genus level. In these cases, bacterial isolates were identified using the conventional culture method at the genus level, not the species level.
^bIf the Difference Value is greater than 0.5, the result is below the level of identification and is not suitable for Tm mapping identification. Details of the identification results for these samples is only included here to demonstrate the accuracy of the Tm mapping method.

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