Table 3 Comparison of the Tm mapping and culture results starting from whole blood samples.

From: Melting Temperature Mapping Method: A Novel Method for Rapid Identification of Unknown Pathogenic Microorganisms within Three Hours of Sample Collection

Bacterial isolates

Tm mapping method

detection

+

Total

Conventional culture method

+

43A(I: 41, NS: 2)

2C

45

27B(I: 18, NS: 9)

128D

155

Total

70(I: 59, NS: 11)

130

200

  1. I: Identified according to the Tm mapping method (Difference Value ≤0.5).
  2. NS: Bacteria were detected, but not suitable for Tm mapping identification (Difference Value >0.5).
  3. A41 Tm mapping identifications matched the culture (or sequencing) results, whereas two samples were not suitable for Tm mapping identification due to the presence of polymicrobial infection.
  4. B18 species could be identified and nine samples were not suitable for Tm mapping identification.
  5. CThese two samples were positive for Staphylococcus epidermidis and Bacillus cereus.
  6. DThese samples were negative using both the Tm mapping method and culture method.