Figure 2 | Scientific Reports

Figure 2

From: Activation of TRESK channels by the inflammatory mediator lysophosphatidic acid balances nociceptive signalling

Figure 2

Regulation of TRESK channels by Gq-coupled LPA receptors heterologously expressed in Xenopus oocytes.

(A) Co-expression of 5-HT2c, bradykinin B1, bradykinin B2, histamine H1 and LPA2 receptors together with TRESK augmented outward currents upon activation with the respective selective agonist. Inset displays representative trace with co-expressed 5-HT2c receptors. (B) Ramp recordings from −100 to +60 mV elicited outwardly rectifying currents amplified after application of LPA and blocked by lamotrigine (100 μM) (C) Dose-response curve of TRESK activation by LPA recorded at a holding potential of +30 mV with a half-maximal activating concentration of 0.2 μM. (D) LPA-induced augmentation of outward currents (upper panel) was abolished either upon incubation with the phospholipase C blocker U73122 (middle trace) or by a mutant lacking the calcineurin binding motif (lower trace).

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