Figure 4

Immunofluorescence staining of TLR4 with typical pain-associated molecules.
Immunofluorescent staining of TLR4 and TRPV1/CGRP/IB4 in both TGs from the AP-1 group with quantification analysis. The results showed that the number of TRPV1/CGRP/IB4 cells was significantly increased in the left TG, compared to the right TG of the AP-1 group. The double labeled staining revealed that increased expression of TLR4 was mainly in the TRPV1- or CGRP- positive neurons, but not in IB4-positive neurons of the left TGs of the AP-1 group (Bar = 40 μm). ***P < 0.01; n = 8 for each group.