Figure 5
From: Induction of p53-independent apoptosis by ectopic expression of HOXA5 in human liposarcomas
Effect of forced expression of HOXA5 on subcellular distributions of RELA in LPS cells.
For panels a and b, T778 cells were transfected with either HOXA5 expression vector or empty vector control (EV) and subjected to immunocytochemistry with RELA antibody 12 h after transfection. (a) Representative immunofluorescent images are shown. Nuclei were stained with DAPI. Arrows indicate the location of RELA puncta. ×40. Size bar = 20 μm. (b) Cell counting summary showing number of cells with nuclear RELA and nucleolar RELA (presence of RELA puncta). Data represent average number of cells in a given field ± standard deviation (SD, error bars). (c) Summary of apoptosis assay results. LPS cells were transfected with either HOXA5 expression vector or EV. Cells were treated with 100 nM leptomycin B (LMB) 9 h after transfection and incubated further for an additional 15 h. Data represent % apoptotic cells ± SD. NS = not significant.
