Figure 2
From: Gene looping facilitates TFIIH kinase-mediated termination of transcription
Rna15 and Ssu72 occupancy of the promoter and terminator regions is adversely affected in the absence of Kin28 kinase activity.
(A) Schematic depiction of a gene indicating the position of the promoter (P), coding (M) and terminator (T) regions amplified by PCR in the ChIP assay. (B,C) Quantification of ChIP signals showing crosslinking of Rna15-TAP or Ssu72-TAP to different regions in kin28-as mutant in the absence of NA-PP1 (-NA-PP1) or in the presence of NA-PP1 (+NA-PP1). Input represents DNA prior to immunoprecipitation. Error bars represent one unit of standard deviation.
