Figure 3
From: Gene looping facilitates TFIIH kinase-mediated termination of transcription
Kin28 occupancy of the promoter and terminator regions is compromised in the absence of Kin28 kinase activity.
(A) Schematic depiction of a gene indicating the position of the promoter (P), coding (M) and terminator (T) regions PCR amplified in the ChIP assay. (B–E) Quantification of ChIP signals showing crosslinking of Kin28 to the promoter, coding and terminator regions of genes in kin28-as mutant in the absence of NA-PP1 (-NA-PP1) or in the presence of NA-PP1 (+NA-PP1). Input represents DNA prior to immunoprecipitation.
