Figure 4
From: Gene looping facilitates TFIIH kinase-mediated termination of transcription
Kin28 physical interacts with Rna15 and Ssu72 in a manner dependent on its kinase activity.
(A) Western blot analysis showing coimmunoprecipitation of Rna15-Myc with TAP-tagged Kin28 in Kin28-as mutant in the absence (-NA-PP1) and presence (+NA-PP1). (B) Western blot analysis showing coimmunoprecipitation of Ssu72-Myc with TAP-tagged Kin28 in Kin28-as mutant in the absence (-NA-PP1) and presence (+NA-PP1). Kin28 was detected by anti-CBP antibodies, while Rna15 and Ssu72 were detected by anti-Myc antibodies. As a control, immunoprecipitation was performed from cells without TAP-tagged Kin28. IP, immunoprecipitation.
