Figure 5
From: Gene looping facilitates TFIIH kinase-mediated termination of transcription
Gene looping is compromised in the absence of Kin28 kinase activity.
Top panels in (A–D) represent a scaled schematic depiction of genes indicating the positions of AluI and NlaIV restriction sites (vertical lines) and the PCR primers (arrows) used for CCC analysis. (A – D), CCC analysis of CHA1, HXT1, ACT1 and ASC1 in kin28-as mutant in the absence of NA-PP1 (-NA-PP1) or in the presence of NA-PP1 (+NA-PP1). P1T1 PCR reflects the looping signal and the C1C2 PCR reflects the loading control indicating that an equal amount of template DNA was used in each CCC PCR reaction. The gel figures shown here are cropped to indicate the bands specific for P1T1 and C1C2 PCR signals used in quantification.
