Table 1 Nucleotide sequences and position of primers used.
From: Isoform-specific interactions of the von Hippel-Lindau tumor suppressor protein
Primer | Position | Direction | Sequence (5’→3’) |
|---|---|---|---|
pGB_VHL30_F | 1–21 | F | ggaggacctgcatatgATGCCCCGGAGGGCGGAGAAC |
pGB_VHL30_R | 619–642 | R | gcatccccgggaattcTCAATCTCCCATCCGTTGATGTC |
pGAD_VHL30_F | 1–21 | F | agattacgctcatatgATGCCCCGGAGGGCGGAGAAC |
pGAD_VHL30_R | 619–642 | R | cacccgggtggaattcTCAATCTCCCATCCGTTGATGTGC |
pGB_VHL19_F | 160–171 | F | ggaggacctgcatatgATGGAGGCCGGGCGGCCGCGG |
pGAD_VHL19_F | 160–171 | F | agattacgctcatatgATGGAGGCCGGGCGGCCGCGG |
pGB_VHL_N_R | 139–159 | R | gcatccccgggaattcTCACTCCTCCTCGGCGCCCAG |
pGAD_p14ARF_F | 1–23 | F | agattacgctcatatgATGGTGCGCAGGTTCTTGGTGAC |
pGAD_p14ARF_R | 376–399 | R | cacccgggtggaattcTCAGCCAGGTCCACGGGCAGACGG |
