Figure 5
From: Selective Generation of Dopaminergic Precursors from Mouse Fibroblasts by Direct Lineage Conversion
Proliferation and self-renewal of iDPs were enhanced by conditional expression of L-Myc.
Schematic diagram of Tet-On/Off advanced system for L-Myc overexpression in iDPs (A). Dox-regulated expression of myc (isoform L-myc) in iDPs by real-time RT-PCR analysis with primers specific for L-myc (see table S1) and GAPDH-specific primer pairs were used for internal control (B). Immunofluorescence staining for Ki67 (red) of cells grown in Dox (+) or Dox (− media for 72 h and nuclei were stained with DAPI (blue) (C, left panel). Results are shown as the percentage of Ki67+ cells of total cells (C, right panel). ##denotes p < 0.001 compared to cells cultured with Dox (−) medium for 72 h. iDPs cultured with Dox (+) or Dox (−) media, respectively, were subjected to immunostaining with Corin and DCX (red) antibodies and nuclear staining with DAPI (blue) (D). (Scale bars: 20 μm)
