Figure 4 | Scientific Reports

Figure 4

From: Sodium-driven energy conversion for flagellar rotation of the earliest divergent hyperthermophilic bacterium

Figure 4

Motility assay of E. coli cells producing MotA and MotB of A. aeolicus in soft-agar plates.

(A) Schematics of primary structures of MotA, MotB of E. coli and A. aeolicus and chimera MotB. We switched the sequence at the middle of the plug region for chimera MotB. (B) and (C) Motilities of E. coli cells in soft-agar plate. MotA and MotB were expressed from two compatible plasmids which were constructed from pBAD24 and pSBETa, respectively, in a E. coli ΔmotAB strain. Plates were incubated at 30 °C for the indicated number of hours. Ec, protein of E. coli; Aa, protein of A. aeolicus; AE, chimera fused proteins of A. aeolicus and E. coli.

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