Figure 2 | Scientific Reports

Figure 2

From: Pathogenicity of Bovine Neonatal Pancytopenia-associated vaccine-induced alloantibodies correlates with Major Histocompatibility Complex class I expression

Figure 2

BNP alloantibodies recognize MHC class I alleles carried by the cell line used for vaccine production.

(a) 29 full length MHC class I clones derived from MDBK cells, the cell line used for the production of Pregsure© BVD, were sequenced and the relative number of clones per identified allele are depicted. (b,c) Full length MDBK-derived MHC class I clones or a mock construct were transfected into HEK293 cells, stained with anti-bovine MHC class I mAb ILA88 as a positive control and IgG isolated from the serum of one (b) or four (c) BNP dam(s) and Ab binding was revealed using flow cytometry. BNP 1 in c is the same dam as in b. Results are based on two separate transfection experiments. aDenotes a local name (Genbank accession number KM397368).

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