Figure 3
MHC class I is a major, but not the only, target of BNP-associated alloantibodies.
(a) Comparison of MHC class I expression and binding of IgG isolated from BNP dams between MDBK cells transduced with a viral TAP inhibitor and MDBK cells that were transduced with a control vector. Data were expressed relative to binding of the control cell line and are average of three separate experiments. MHC class I expression was measured using mAb ILA88. (b) Comparison of MHC class I and B2M expression and binding of IgG isolated from BNP dams between wild-type MDBK cells (Control) and B2M KO MDBK cells. Data expressed as in A. B2M expression was measured using mAb B1.1G6. (c) Wild-type MDBK cells (Control) and B2M KO MDBK cells were stained with sera from Pregsure© BVD-vaccinated BNP (BNP+Vacc+, n = 7) and non-BNP (BNP−Vacc+, n = 7) dams. Alloantibody binding on wild-type and B2M KO MDBK cells was compared using a paired t-test. (d) Data from c were transformed to express Ab binding to B2M KO MDBK relative to wild-type MDBK and relative alloantibody binding was compared using an unpaired t-test for equal variance. In all experiments Ab binding was measured using flow cytometry and respective isotype controls were subtracted. GMFI = Geometric mean fluorescent Intensity.
