Figure 4

Effect of APAP and indomethacin in hyperthermic Trpa1^−/−/Trpv1^−/− mice.

(A–C) Administration of yeast (2 g/kg) produced hyperthermia in wildtype and Trpa1^−/−/Trpv1^−/− mice. (A) APAP (300 mg/kg) evoked hypothermia in wildtype mice, but only restored normal body temperature in Trpa1^−/−/Trpv1^−/− mice (n = 6). (B) The antipyretic indomethacin (30 mg/kg) restored pre-yeast injection temperature in wildtype and Trpa1^−/−/Trpv1^−/− mice (n = 6). In Trpa1^−/−/Trpv1^−/− mice, this was later followed by a slow reduction in body temperature. (C) Adminstration of APAP (300 mg/kg) 1h after indomethacin (30 mg/kg) rapidly elicited hypothermia in wildtype, but not Trpa1^−/−/Trpv1^−/− mice (n = 6). The slow reduction in body temperature in Trpa1^−/−/Trpv1^−/− mice was not different from that seen in (B) in animals receiving vehicle instead of APAP. Data are mean ± sem; *p < 0.05, **p < 0.01, ***p < 0.001 compared to pre-yeast injection and ^†p < 0.05, ^††p < 0.01, ^†††p < 0.001 compared to Trpa1+/+ (ANOVA followed by Tukey’s HSD test).