Figure 3
Validation of the aggregated rank based on multiple omics data.
The heatmap shows relative ranks for all DMRs across all 15 possible ranks (A). DMRs are ordered according to the rank aggregated from all data resources. The color red indicates higher relative ranks. All DMRs ranked to the first position in each possible aggregated rank are also shown (B) with the UCSC genome browser plot in the black box. Prioritized features, such as TFBS and conservation and dynamics of DNA methylation in DMRs are provided in browser pictures. Functional elements defined by chromatin segments are also exhibited. The ranks of DMRs were re-mapped to known methylated CRC genes and then the significance of the average relative rank in contrast to the random distribution for all possible ranks was determined (C). The y-axis shows the minus log-transformed p values. Functional enrichment result with GO for the top 5% DMR-coupled coding genes is plotted with significant p values on the x-axis (D). The red bar represents significant GO terms tightly associated with disease status. Validations characterized by chromatin modification alterations are also plotted with minus log-transformed p values on the y-axis (E). The red bars indicate comparisons between top- and tail- ranked 5% DMRs, while the blue bars compare the number of significant DMRs between top- and tail-ranked 5% DMRs. The black line indicates the significance cutoff of 0.05.
