Figure 5 | Scientific Reports

Figure 5

From: IRF6 is the mediator of TGFβ3 during regulation of the epithelial mesenchymal transition and palatal fusion

Figure 5

Ectopic expression of IRF6 enhances palatal fusion and SNAI2 expression.

Palatal shelves from E13.5 mouse embryos were infected with control adenovirus (AdIE) (n = 6) or with AdIRF6 (n = 6). (a) At 24 or 48 hours after infection, IRF6 were detected by immunohistochemistry staining. Expression of SNAI2 and TWIST was shown by immunofluorescence. Nuclei were counterstained with Hoechst stain. → : MEE. (b) 24 hours after infection, expression of Plakophilin and ZO-1 were detected by immunofluorescence. The scale bar is 20 μm. (c) Quantification of staining intensity of IRF6, SNAI2 and TWIST in the MEE at 24 hours after adenovirus infection. Statistics analysis was performed by t-test. Error bars represent s.d. *p < 0.001; n.s. not significantly different.

Back to article page