Figure 6
Ectopic expression of IRF6 rescues shTgfβ3-blocked palatal fusion.
Palatal shelves from E13.5 mouse embryos were infected with (a) control lentivirus (shLuc) (n = 9), lentivirus carried shTgfβ3 (n = 10), AdIRF6 (n = 11) or AdIRF6–R84C (n = 6); (b) control lentivirus (shLuc) (n = 4), lentivirus carried shTgfβ3 (n = 4), lentivirus carried shSnai2 (n = 4), AdIRF6 (n = 4), AdIRF6 and shTgfβ3 (n = 4), or AdIRF6 and shTgfβ3 and shSnai2 (n = 4). At 48 hours after infection, IRF6 were detected by immunohistochemistry staining. Expression of SNAI2 and TWIST was shown by immunofluorescence. Nuclei were counterstained with Hoechst stain. shSnai2 lentivirus infection abolished 93% of SNAI2 expression. → : MEE. The scale bar is 20 μm. (c, d) Quantification of the degree of fusion of the palatal shelves. Statistics analysis was performed by t-test. Error bars represent s.d. *p < 0.05, **p < 0.01; n.s. not significantly different.
