Figure 2
Systems level analysis of mpkDCT cells.
(A) Total proteins or phosphoproteins identified. Of the 6330 proteins identified, 3270 had at least one phosphorylation site. (B) Total phosphosites identified and the number of unique sites not published in the PhosphoSitePlus database. (C) Correlation between mpkDCT proteome and a published DCT transcriptome. Normalized spectral count of each protein from the current mpkDCT proteome was plotted against average transcript signal of that particular protein from a published DCT transcriptome19. Linear regression was applied and correlation coefficient calculated. (D) mpkDCT proteome PANTHER GO-term molecular function analysis. Next level analysis of the molecular function subgroups of binding, transporter activity and receptor activity. (E) Venn diagram comparison of current mpkDCT proteome and reported mouse kinome (http://kinase.com/kinbase). (F) Comparison of the extracted mpkDCT kinome and kinases identified in a DCT transcriptome19.
