Figure 6
Ex vivo studies on isolated cortical tubules from mouse kidney highlight a role for CAMKK and PI3K in VP signaling in mpkDCT cells.
Top panels are representative immunoblots and the bottom panels are summarized data. In all experiments, dDAVP (10−6 M) treatment for 20 min significantly and consistently increased phosphorylation of NCC at thr-58 (surrogate marker of NCC activation). (A) Effects of the CAMKK inhibitor STO609 on baseline and dDAVP stimulated NCC phosphorylation. (B) Effects of the PI3K inhibitor LY294002 on baseline and dDAVP stimulated NCC phosphorylation. (C) Effects of the PKA inhibitor H89 on baseline and dDAVP stimulated NCC phosphorylation. *represents significant difference from control group, #indicates significant difference between groups as indicated.
