Figure 2

Optimization of 2D-FsLDW and characterization of FsLDW-fabricated BSA/RhB hydrogel micro/nano-structures by confocal microscopy, SEM and optical microscopy (OM).

(a) The 532-nm-excited 3D-reconstructed confocal microscopic fluorescent image of three BSA/RhB hydrogel micro-squares with different FsLDW scanning steps. Scale bar, 10 μm. (b) Y-Z-plane cross-section confocal microscopic fluorescent image of (a). Scale bar, 1 μm. (c) Confocal microscopic fluorescent (left in each column) and bright-field (right in each column) top-view images of BSA/RhB micro-squares in air FsLDW-fabricated with different scanning step and laser power. Scale bar, 10 μm. (d) Confocal microscopic fluorescent images in water (1 and 2, scale bar, 10 μm) and SEM image in vacuum of a BSA/RhB hydrogel spiral nano-line (3, scale bar, 1 μm). (e) 1, optical microscopic image of a BSA/RhB hydrogel relief micro-sculpture of roses; 2, fluorescent images of “golden” roses in 1 after filtering sub-565-nm light including 532-nm excitation light; scale bar, 10 μm.