Figure 5 | Scientific Reports

Figure 5

From: Lycorine suppresses RANKL-induced osteoclastogenesis in vitro and prevents ovariectomy-induced osteoporosis and titanium particle-induced osteolysis in vivo

Figure 5

Anisomycin (ANI) attenuated the inhibitory effect of LY on osteoclastogenesis in vitro.

(A) BMMs were stimulated with 30 ng/mL M-CSF, 50 ng/mL RANKL and LY (0, 0.1, 0.2 or 0.4 μM), with or without 2.5 pg/mL ANI, for 5 days. All cells were fixed and stained for TRAP as described in the Methods section. (B,C) The number and areas of TRAP-positive cells were determined using Image J (*P < 0.05; **P < 0.01). (D) BMMs were treated with 0.4 μM LY for 4 h and then treated with 100 ng/mL RL, with or without 2.5 pg/mL ANI, for 5 min. Cell lysates were analysed using western blotting. The expression of phosphorylated P38 and JNK, total P38 and JNK and β-actin was evaluated.

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