Figure 3 | Scientific Reports

Figure 3

From: Reduced Expression of Argonaute 1, Argonaute 2 and TRBP Changes Levels and Intracellular Distribution of RNAi Factors

Figure 3

Relative levels of RNAi factors between the cytoplasm and the nucleus in T47D cells.

(A) Western blot data showing relative levels of Dicer, TNRC6A, AGO1, AGO2 and TRBP between the cytoplasm and the nucleus in siCtrl-, siAGO1, siAGO2, or siTRBP-treated cells. [dsRNA] = 25 nM. The cytoplasmic and nuclear fractions were prepared in the equal amount of buffers. A equal volume of samples from each fraction were analyzed by SDS-PAGE to evaluate relative levels of each protein between the cytoplasm and the nucleus. Data were representative from three independent experiments. (B) Ratio of each RNAi factor between the cytoplasm and the nucleus after siCtrl, siAGO1, siAGO2, or siTRBP treatment. Data were averaged from 9 data sets for siCtrl-treated samples and 3 data sets for siAGO1-, siAGO2-, or siTRBP-treated samples.

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