Figure 2
From: Estrogen deficiency heterogeneously affects tissue specific stem cells in mice
The expression of CXCR4 and intracellular ROS levesl in bone marrow mononuclear cells (BM-MNCs).
(A) The bone marrow was collected from the femur and tibia 2 months after treatments and the number of total collected mononuclear cells from each mouse was directly counted. (B) The expression of CXCR4 was detected in freshly collected BM-MNCs by flow cytometry. (C) The intracellular ROS level was measured as the mean fluorescence intensity in the freshly collected BM-MNCs after 30 min loading with 10-μM CM-H2DCFDA.
