Figure 6
High-affinity VLA-4 epitope expression on infected monocytes.
For single-color staining, 1 × 106 monocytes were cultivated in medium alone or in medium containing Leishmania. The cells were resuspended in medium alone (A) or in medium containing Mn++ (B) The cells were then stained with an anti-VLA4 high-affinity epitope antibody and analyzed by flow cytometry. Empty histograms cells incubated with isotype control antibody. Grey histograms cells incubated with the anti-VLA4 high affinity epitope antibody. Scatterplots: Each pair of data connected by line represents an independent experiment using the same batch of monocytes, cultured with medium alone (Control) or with medium containing Leishmania (Infected) (Mann-Whitney test).
