Figure 3

Removing RPM by MCS.

(A) RPM contaminations in CD11c⁺ cell isolates obtained without RPM-depletion or after RPM-depletion on the indicated column. (B) Relative yield of splenic DCs after RPM-depletion using the negative selection column compared to isolation without depletion. (C) RPM contaminations in CD11c⁺ cell isolates obtained without RPM-depletion or after RPM-depletion using the indicated protocol. (D) Relative yield of splenic DCs after RPM-depletion using the two antibody-based depletion protocols compared to isolation without depletion. (D) RPM contaminations in B or T cell isolates with or without RPM-depletion using the F4/80-bead protocol. (E) CD11c⁺ cell isolates obtained without (left tube) or with RPM depletion using the 3-antibody protocol (right tube). Results are shown for one representative of two to three individual experiments using 2–4 mice per group. Error bars, s.d. (n = 2–4 mice); *p < 0.05; ***p < 0.001.