Figure 4

Functional consequences of RPM contaminations.

(A) IL-6 concentrations in supernatants from B cell (left graph), DC (middle graph) or T cell (right graph) cultures obtained by MCS from spleens or the mesenteric lymph nodes with or without RPM-depletion using the 3 antibody-based protocol. (B) mRNA levels of MR determined by RT-PCR. Cell type, source tissue, SpiC-expression and RPM-depletion as indicated. (C) mRNA-expression of GITRL (left graph) or PPARγ (right graph) in CD11c⁺ cell isolates with or without RPM-depletion using the 3 antibody-based protocol. (D) mRNA-expression of GITRL (left graph) or PPARγ (right graph) in sorted RPM, CD11c⁺ CD8⁺ DC or CD11c⁺ CD8⁻ DCs. (E, F) γδ T cells stained for the RPM-Marker MR (E, black line) or CD163 (F, black line) or the corresponding isotype (grey background). Left histograms: marker expression without excluding RPM, right histograms: marker expression when the analysis gate is free of autofluorescent RPM. The average (mean) values ± s.e.m. are shown. Results are shown for one representative of two to three individual experiments using 2–4 mice per group. Error bars, s.d. (n = 2–4 mice); *p < 0.05; ***p < 0.001.