Figure 7
From: Subcellular and Dynamic Coordination between Src Activity and Cell Protrusion in Microenvironment
The correlation of Src activity and boundary translocation at the edge of non-polarized cells.
The time courses of normalized ECFP/FRET emission ratio sampled at (A) the protrusive edge, or (B) non-protrusive edge, of multiple cells; the time course of translocation sampled at (C) the protrusive edge, or (D) non-protrusive edge, of multiple cells. (E) The line scans of ECFP/FRET ratio at the protrusive (P) and non-protrusive (NP) edges are compared before and after peeling-off the gel membrane; (F) The line scans of boundary translocation at the protrusive (P) and non-protrusive (NP) edges are compared before and after peeling-off the gel membrane. The temporal CC at (G) the protrusive edge, or (H) non-protrusive edge, of multiple cells. Grey dots-data; black solid lines with dots-the average curve. (I) Statistical comparison of the minimal values on the temporal CC curve between Src activity and cell boundary translocation; (J) Statistical comparison of the time lag values on the temporal CC between Src activity and cell boundary translocation. *indicates significant difference by T-test, one-tailed, number of cells = 3, number of samples = 40, p-value < 0.05.
