Figure 1
From: Deletion of the tyrosine phosphatase Shp2 in Sertoli cells causes infertility in mice
Shp2 depletion in Sertoli cells impeded testes development in mice.
(A) Shp2 expression was detected with immunofluorescence staining in the seminiferous tubules at embryonic day 18 (E18). Arrowheads indicated Sertoli cells. The cell nucleus was stained with DAPI. (B) Shp2 protein levels were analyzed by Western blotting in primary Sertoli cells and germ cells isolated from 14-day-old mice. Wt1 is a marker of SCs and SCP3 is a marker protein of GCs. Tubulin was used as a loading control. The full-length blots are presented in Supplemental Figure S6. (C) The stereomicroscopy images of the testes from Shp2f/f and SCSKO male mice at 4 weeks. (D) The average weight of the testes from 2-, 3- and 4-week-old Shp2f/f and SCSKO mice. The values are expressed as the mean ± standard error of the mean (SEM) from 8 mice. Statistical analysis was performed using Student’s t-test. Asterisks denote the statistical significance, **P < 0.01; ***P < 0.001.
