Figure 3
From: Deletion of the tyrosine phosphatase Shp2 in Sertoli cells causes infertility in mice
BTB integrity was disrupted by Shp2 deletion.
(A) BTB permeability was assayed with the biotin tracer (red) using confocal microscopy in two-week-old mice. Asterisk indicates the biotin tracer in the adluminal compartment of the seminiferous tubules. (B) Immunohistochemical staining of the BTB junction proteins Cx43, Claudin11 and JAMA in seminiferous tubules in two-week-old mice. (C) Altered BTB-related genes in the microarray gene database and the protein levels of various genes were analyzed by Western blotting in testes from two-week-old mice. The full-length blots are presented in Supplemental Figure S7. (D) The quantity of the cell junctions between primary Sertoli cells isolated from two-week-old mice was measured by transepithelial resistance (TER) assays. The experiments were repeated at least three times and one representative result was shown. (E) Shp2 expression and Erk and Akt phosphorylation in primary Sertoli cells used in the TER assays. Tubulin was used as a loading control. The full-length blots are presented in Supplemental Figure S8. Ad, adenovirus virus; f/f, Shp2f/f cells; ko, SCSKO cells; Q79P, constitutively active Shp2 mutant. The values are expressed as the mean ± SEM. Statistical analysis was performed using Student’s t-test. Asterisks denote the statistical significance, **P < 0.01; ***P < 0.001.
