Figure 4
From: Deletion of the tyrosine phosphatase Shp2 in Sertoli cells causes infertility in mice
Shp2 deficiency decreased the number of SSCs and initiated the untimely and excessive differentiation of SSCs in testes.
(A) Undifferentiated SSCs in the seminiferous tubules were identified by PLZF-positive immunofluorescence staining (green). (B) Proliferating SSCs in the seminiferous tubules were identified with PLZF and PH3-double positive immunofluorescence staining (yellow). Asterisks indicated positive SSCs (yellow). (C) Quantification of the proliferating SSCs (PLZF and Ph3 double stained cells) per tubule in the intersecting surface. The values are expressed as the mean ± SEM from at least 5 mice from different litters. Statistical analysis was performed using Student’s t-test. Asterisks denote statistical significance. **P < 0.01. (D) The differentiated SSCs in the seminiferous tubules were identified by c-Kit-positive immunofluorescence staining (green). The cell nucleus was stained with DAPI. The experiments were repeated at least three times and one representative result is presented.
