Figure 5
From: Deletion of the tyrosine phosphatase Shp2 in Sertoli cells causes infertility in mice
Shp2 deletion enhanced spermatogonia differentiation and altered the expression of proteins essential for SSC maintenance.
(A) The differentiated spermatogonia in the seminiferous tubules are denoted by stra8-positive immunofluorescence staining (green). (B) The spermatocytes were identified as Scp3 positive (red). Asterisks denote statistical significance. The cell nucleus was stained with DAPI. Tubulin was used as a loading control. The experiments were replicated at least three times and one representative result is presented. *P < 0.05: **P < 0.01; ***P < 0.001. (C) Altered genes related to SSC maintenance in the microarray gene database and the protein level of some of these genes was analyzed by Western blotting in primary Sertoli cells at postnatal day 10. The full-length blots are presented in Supplemental Figure S9.
