Figure 6
From: Deletion of the tyrosine phosphatase Shp2 in Sertoli cells causes infertility in mice
The clone formation ability of SSCs seeded on primary Sertoli cells was impaired by Shp2 deletion in Sertoli cells.
(A) The clone formation of SSCs co-cultured with primary Sertoli cells isolated from 2-week-old mice. The arrowhead indicates the SSC clones. (B) Quantification of the number of stem cell clones identified by Thy (CD90) staining and flow cytometry. Ad, adenovirus; Q79P, constitutively active Shp2 mutant. The experiments were replicated at least three times and one representative result is presented.
