Figure 3

The luciferase activity of the three constructs transfected in HEK293 were graphed after normalization against the plasmid pRL-SV40 expressing the Renilla luciferase gene.

Data of the control plasimid (contained the pGL3 promoter, rather than the GRIN2B 3′UTR.) is presented as mean with SEM of n = 6 samples. Data of both 3′UTR constructs (pGL3-promoter-A and pGL3-promoter-C) are presented as means with SEM of n = 5 sample espectively. Comparisons were determined using ANOVA with post-hoc comparisons ***p < 0.001, One-way ANOVA test. Error bars are standard error.The luciferase activities of 3′-UTR constructs were significantly (p < 0.001) reduced compared with the pGL3 -Promoter vector 24 h after transfection, A mutant-type 3′-UTR construct (pGL3-promoter-C) showed lower (p < 0.001) activity than the wild-type 3′-UTR construct (pGL3-promoter-A). The relative firefly luciferase activities were normalized against the Renilla luciferase activities.