Figure 1
From: Enhancer of zeste homolog 2 silencing inhibits tumor growth and lung metastasis in osteosarcoma
EZH2 is overexpressed in human osteosarcoma cell lines.
(A) EZH2 mRNA expression was determined in hFOB1.19 osteoblasts, MTF osteoblasts and osteosarcoma cell lines (SaOS2, U2OS, MG63 and HOS) by qRT-PCR analysis. EZH2 expression was significantly increased in MTF cells compared with hFOB1.19 osteoblasts, whereas TSSC3 expression was notably decreased. Values shown are the mean ± SD from three wells from a representative of three independent experiments. ***P < 0.001; **P < 0.01; bars, SD. (B) Western blot analysis showing EZH2 and TSSC3 expression in hFOB1.19, MTF and osteosarcoma cells (upper). The results were consistent with that of qRT-PCR. Blots are representative of three experiments and were reprobed for β-actin to verify equal loading (lower). Band intensities were measured by densitometry and normalised to β-actin expression. (C) Osteosarcoma cell lines were immunostained with goat anti-EZH2 (red) and rabbit anti-TSSC3 antibody (red) and Hoechst33258 (blue) for observation by laser confocal microscopy. Scale bars: 50 μm.
