Figure 4 | Scientific Reports

Figure 4

From: Two single amino acid substitutions in the intervening region of Newcastle disease virus HN protein attenuate viral replication and pathogenicity

Figure 4

Growth kinetics of rescued wt and HN-mutated NDV viruses.

The growth characteristics of viruses were determined by means of single and multi-cycle growth curves in both BHK-21F cells (a) and DF1 cells (b) infected with viruses at MOIs of 5 and 0.01, respectively. Supernatants were collected at 8-h or 12-h intervals. Viral titers were quantitated in SPF embryonated chicken eggs. The data shown represent three independent experiments; bars represent the mean ± SD of three independent experiments (n = 3) (*P < 0.05, ***P < 0.001).

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