Figure 3 | Scientific Reports

Figure 3

From: Transcriptomic and metabolomic analyses identify a role for chlorophyll catabolism and phytoalexin during Medicago nonhost resistance against Asian soybean rust

Figure 3

Expression profiles of genes involved in phenylpropanoid and flavonoid pathways in M. truncatula inoculated with P. pachyrhizi. M. truncatula leaves were inoculated with P. pachyrhizi at 12, 24, 48 and 72 hours post-inoculation (hpi) or mock control (0 time). Expression profiles are presented for genes encoding Phenylalanine ammonia-lyase (PAL), Chalcone synthase (CHS), Chalcone reductase (CHR), Chalcone isomerase (CHI), Isoflavone synthase (IFS), Isoflavone 4′-O-methyltransferase (HI4′OMT), 2-Hydroxyisoflavanone dehydratase (2HID), Isoflavone 2′- hydroxylase (I2′H), Isoflavone reductase (IFR) and Vestitone reductase (VR). The expression of genes was evaluated by RT-qPCR with gene-specific primer sets (Supplementary Table S10). The values represent the fold induction compared to mock control. Three biological replicates were used for each inoculation. The Ubiquitin gene was used as an internal control. Bars represent the means ± standard deviation (SD).

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