Figure 3 | Scientific Reports

Figure 3

From: Curcumin analogues as selective fluorescence imaging probes for brown adipose tissue and monitoring browning

Figure 3

Lead optimization and in vivo NIR imaging.

(a) The structure and synthesis of CRANAD-29. (b) Excitation and emission spectra of CRANAD-29. (c) Two-photon microscopic imaging of 3T3-L1 cells with CRANAD-29 (left: red—CRANAD-29 signal, middle: green – autofluorescence of the cells, right: merged). The images clearly demonstrated that CRANAD-29 was accumulated in the oil droplets. Scale bar: 50 micron. (d) Representative in vivo NIR image of a CRANAD-29 injected mouse. The two lobes of the interscapular BAT can been clearly seen. (e) Stepwise dissection and validation of BAT signal after CRANAD-29 injection. There was no significant signal decrease after skin and WAT removal (middle), while the signal disappeared after BAT removal (right). (f) Comparison of F(BAT)/F(intact) ratio for CRANAD-2, -3 and -29. The ratio for CRANAD-29 was much higher than that for CRANAD-2 and -3, indicating that the selectivity of BAT over WAT was significantly improved after the lead optimization.

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